FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609815
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG203380.5634083741133549No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG98760.2735874276105562No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC70910.19643665949640077No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC66440.18405375344719882No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG61850.17133842038996458No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG56090.15538192400441575No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG53030.14690503529959292No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA46400.12853844310581014No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44470.1231919087266245No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC43600.12078181291839056No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA41250.11427178401109198No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC40700.11274816022427742No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG40330.11172317694951125No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39780.1101995531626967No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG38010.10529625479422076No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37610.10418816476744654No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT37000.10249832747661583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG3950.034.957856
CGCGGCA64750.034.93375832
ACGATCG501.8555985E-434.5208746
AGGACCG7900.034.44879570
GCGGCAC66550.034.05373833
CCGTCGC64850.033.02520845
CGGCACC69100.032.81029534
CCCGTCG65100.032.77809544
TCGCCGG65250.032.57087748
GCACCCC71400.031.81788436
GTGCCGT6800.031.0775811
CACCCCC74150.030.55792837
GTACGTA2750.030.14612220
TCGCCGT5300.030.0682561
GTTCCGT2550.029.8888191
CCCCCGA77000.029.64731219
GTACGGC8750.029.58932314
TACGGCC8800.029.42160815
GTCGATT5550.029.25864621
CGCCCTA16450.029.14094761
Produced by FastQC (version 0.11.5)