FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3445812
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG243750.7073804374701812No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG110900.3218399610889973No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG66560.1931620181251908No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC63030.1829176983538278No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58920.17099017590048443No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC51050.14815085675016512No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC46620.13529467074814297No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45700.1326247630456914No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG40660.11799831215400028No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC39560.1148060312054169No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA37700.10940817432872137No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG37530.10891482181848575No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG35700.10360402714947885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA2200.037.555618
CGCGGCA73300.037.52769532
TTATCGG2350.036.623387
GCGGCAC75050.036.43595533
GTTCCGT4850.035.6149441
TCGCCGG73200.035.32077848
CGGCACC78300.035.0289134
CCGTCGC76350.034.20121845
AGGACCG8800.033.84156870
CCCGTCG77950.033.7059644
GCACCCC81800.033.56349636
GTTATCG2600.033.10196
GGCACCC85850.031.84593835
CACCCCC85800.031.8177137
TAGTCCG4350.031.6560696
CCCCCGA88150.031.025919
TATCGCG450.004672797430.60086610
AGGAGGG123600.029.555770
GTCCGTT2750.028.7934682
ACCCGCG95600.028.72819925