FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3445812
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC142790.41438708786201917No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC118430.3436925752188454No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT71800.20836888373480617No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC70420.20436402218112887No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG61480.17841948428991483No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA59570.17287652373373824No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58440.16959718057746623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT64450.052.960459
CCGAACT10450.052.3968567
TGGCGCG70000.048.90819515
CGCGGTG70150.048.80432510
CGAACTT11750.047.7570768
GCCGGTT11350.046.6867943
CGGTTCA11650.045.779255
CCGGTTC12700.041.723424
GTAGTCC82900.041.3860727
TGTAGTC83250.041.27694326
CGTGCCT82850.041.23212420
CTACTCG83950.041.13722637
TAGTCCC83550.041.03654528
TACTCGG84400.040.93768338
CCTGTAG86300.039.90666624
GCCGAAC14050.039.53463466
CTTAGGC13600.039.2307118
ACTCGGG90300.039.135639
CCTTAGG14000.038.59259417
GCGTGCC91400.037.51540819