FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434809
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC141230.4111727901027393No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC115200.33538982808068807No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT70600.20554272450083833No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC68850.20044782693884872No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN59730.17389613221579425No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG59710.1738379048150858No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA59040.17188728689135263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT65000.053.3365449
CCGAACT10800.050.58667
TGGCGCG69250.050.1545615
CGCGGTG70750.049.2937410
CGGTTCA11300.047.8346565
GCCGGTT11900.045.4148753
CGAACTT12600.045.07099568
CCGGTTC13050.041.412194
GTAGTCC85650.041.1739427
TGTAGTC85750.041.1066726
CGTGCCT84950.041.05414220
TAGTCCC86150.040.9517528
TACTCGG85450.040.77472738
CTACTCG85700.040.68131637
CCTGTAG87350.040.3318824
CTTAGGC13500.040.0569118
ACTCGGG90800.039.3103339
CGGCAAA6750.039.0669021
GGGGTAG15450.038.8523061
AGTCCCA92500.038.11930529