FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334569
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113640.34079366778735126No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC67830.20341459420992639No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA58460.17531501072552405No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC57400.1721361891146952No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT34440.10328171346881711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT36600.046.4593129
TGGCGCG38300.044.57080515
CGGCATA2750.043.8334431
CTACTCG44150.039.22670737
TACGGCC4300.039.0660747
TCTACGG4400.038.956775
TACTCGG44600.038.93602838
GTCTACG4350.038.616464
CCGAACT6800.037.4915667
CGCGGTG46000.037.2635610
GTAGTCC46550.037.0210727
TAGTCCC47000.036.83891728
ACTCGGG48900.036.17679639
GTACGCA3950.036.11713465
TGTAGTC48450.035.83321426
GGGGTAT12800.035.7858961
GATCGCT47600.035.50169861
GCCGGTT7400.035.2047923
AGGATCG48100.035.1227259
CGGGTAG5450.034.7565271