FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3324371
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113670.3419293454310605No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC65530.1971199965346828No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC56610.17028785295022728No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA53580.1611733467774806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT36100.047.967939
TGGCGCG38550.044.46792215
CCGAACT6500.043.58524367
CGGCATA2450.042.325111
GCGGTAT2100.041.149411
CTACTCG43500.039.9735937
TACTCGG44200.039.13665438
GCCGGTT6950.038.9711763
CGGTTCA7050.038.910585
GTCTACG4350.038.619044
CGCGGTG45800.037.8830810
GTAGTCC46900.037.76878427
TAGTCCC47050.037.5979528
ACTCGGG47550.036.99209239
CGGGTAG4900.036.6817631
CGGCAAA7450.036.653361
TGTAGTC48950.036.30085826
CGTGCCT49050.035.0058720
CCTGTAG50050.034.98692324
AGGATCG48250.034.92212759