FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315273
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG154660.46650758474490633No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG129030.3891987175716751No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC74800.22562244496908704No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT71500.215668513573392No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT60970.18390642339258337No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58380.17609409541838636No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA52350.1579055480498891No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG51310.1547685514888216No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC49860.15039485436041014No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT47220.14243170924385412No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA46320.13971700068139184No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45480.13718327268976038No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG45050.13588624526547285No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT41050.12382087387675163No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG37320.11256991505676908No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG35040.10569265336519797No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34360.10364154022911537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA49250.037.21504232
GCGGCAC51400.035.6702733
CGCGATT400.00252601434.6956236
CGGCACC53050.034.57549734
CTTACCC12850.032.815570
AGGACCG6000.032.17632370
GCACCCC57900.031.63953436
TCGCCGG51100.031.59902248
ACCCGCG58550.031.43187125
CCCGTCG52500.031.02230844
CCGTCGC52350.030.92211345
CCCCCGA59650.030.87801219
AGTCCGC60700.030.6091087
CACCCCC60150.030.52248837
CAGTCCG57950.030.393026
CCCCGAC61450.029.97987620
TCCGCCC63550.029.942859
GGCACCC63350.029.12693835
GTCCGCC64950.028.8188928
CCGCGCG64500.028.4319627