FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300409
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA176790.5356608832420466No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC106650.3231417681869126No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC95230.2885399961035132No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT48170.1459516078158798No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA37170.11262240528370877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT50500.053.412429
TGGCGCG53900.049.91351315
CGCGGTG55450.048.45746210
CCGAACT8300.047.0263167
GCCGGTT8300.045.77383
GGGGACT44950.045.76681
ACTCTTC47800.044.509085
GGACTCT49450.044.2818073
CGGTTCA8600.044.176375
TCTGGTC47500.043.770910
CGGGACT11550.043.3250471
GGGACTC49500.043.1206862
CGTGCCT63350.043.03977620
CTACTCG63700.042.47878337
TACTCGG64050.042.37047238
GTAGTCC64450.041.9833827
TAGTCCC65050.041.65692528
TGTAGTC65400.041.47366726
CCGGTTC9200.041.29534
TTCTGGT50850.041.023119
Produced by FastQC (version 0.11.5)