FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296835
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA169660.5146147744730931No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC104020.31551472851992896No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC91960.27893418991244634No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT46010.1395580913209184No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA36760.11150087887322234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT50250.051.770119
TGGCGCG53350.048.75450515
CGCGGTG54950.047.78219210
CCGAACT8600.047.4222767
GCCGGTT8500.046.3290443
TCTGGTC45950.045.7128410
CGGTTCA8700.045.6679955
GGGGACT45750.045.4242821
ACTCTTC47450.044.2691045
GGACTCT49400.043.2144433
TTCTGGT49000.042.6559379
CTACTCG61100.042.5962137
TACTCGG62400.042.000338
CGTGCCT62900.041.76198220
CGAACTT9950.041.7199868
CTGGTCC50300.041.6221811
GTAGTCC64500.041.57532527
TAGTCCC64300.041.3874328
GGGACTC51650.041.274442
CGGGACT10300.041.231341
Produced by FastQC (version 0.11.5)