FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3395226
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN298200.8782920488945359No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG155910.45920359940693195No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG58810.17321380078969706No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG48700.1434366961138964No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG42280.1245277928479577No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC40110.118136465731589No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT34670.1021139682601394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA46500.037.3818732
GCGGCAC48400.035.87658333
TCGCCGG47000.035.41252548
CCCGTCG48500.034.7226144
CCGTCGC48750.034.30555345
CGGCACC51750.033.59119834
TCGTATG4000.032.25082440
TCGTATC3550.032.15424333
CCCCCGA55850.030.9579619
ACCCGCG57000.030.43908725
CCCCCGT55100.030.30052842
GCACCCC59350.029.3009736
CCCCGAC58850.029.23166520
CCGCGCG60200.028.94045427
AATTCGT4450.028.66592430
CGTATGC5000.028.61215241
GCGCGGG60550.028.46923462
CGCGCTA854.525389E-628.33528528
CACCCCC61400.028.30091737
CGTCGCC59700.027.98718546