FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379867
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN295250.8735550836763695No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG157930.4672669072481255No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG56190.16624914530660526No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG49010.14500570584582176No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40920.12106985274864365No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC40450.11967926548589042No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT34070.10080278306809114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG4500.038.5640636
CGCGGCA48000.037.06485732
TCGTATC4650.035.70200733
GCGGCAC50050.035.5883233
TCGTATG5100.035.53835340
ACGAATT5250.034.66490627
AATTCGT5100.034.45830
TCGCCGG49150.034.08782648
CGTATGC5450.033.9335641
CCCGTCG50800.033.5030144
CCGTCGC50450.033.35899745
CGGCACC54100.032.76670534
CGAATTC5900.032.63029528
TATGCCG5900.032.0104643
GAATTCG5450.031.58080329
ACCCGCG57600.030.99361225
GTTATCG2600.030.0533626
GCACCCC59600.029.99067736
CCCCCGA59250.029.96534219
CGTATCT5550.029.943934