FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379867
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN285170.843731424934768No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA74320.2198903092932355No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC58860.17414886443756514No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC45870.13571539945210861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT29850.048.2578059
TGGCGCG32600.044.2905415
CGCCGTA3450.042.12797553
TACGGCC3750.041.675967
CTACTCG37000.040.37146837
CCGAACT4950.040.28967
CGGTATA1550.039.7043081
GTCTACG3850.038.8285374
TACTCGG38450.038.801338
CGCGGTG37750.038.78880710
GCCGTAT3800.038.3002654
CGGTAAG3050.038.1132431
CGGCAAT4400.038.0750271
TAGTCCC40100.036.86006528
TCTACGG4100.036.460945
GTAGTCC40400.036.28780727
ACTCGGG42050.036.1902639
AGGATCG39000.036.17632759
GGGGTAG15550.035.838851
GATCGCT39400.035.80999461