FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375742
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN284930.8440514707581327No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA74050.2193591808852691No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC59630.1766426462685833No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC44710.13244495580527185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT31700.047.596469
TGGCGCG32450.046.80775515
CTACTCG37000.042.15022337
TACTCGG37500.041.63208438
CGCGGTG38050.039.83190510
AGGATCG38900.038.6470859
CGGTCTA1950.038.5832331
ACTCGGG41000.038.2131739
CGGTACG901.3460522E-1037.998641
CGGGTAG5500.037.9295541
CCGAACT5900.037.46942567
GTAGTCC42100.037.10291727
GATCGCT40600.036.94225761
GCCGGTT5650.036.6892473
CGTGCCT42350.036.49429320
TAGTCCC43050.036.3313128
GGGGACT33800.035.5141141
TGTAGTC44200.035.3814726
AGTACGC4950.034.83743764
CCTGTAG45350.034.65678424
Produced by FastQC (version 0.11.5)