FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359274
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN292880.8718550496327481No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG158250.4710839306350122No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG55990.16667291801740494No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG49620.14771048744460857No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG43240.12871828853496323No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC40320.12002593417506283No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT34750.10344497055018435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3850.038.03973840
CGCGGCA46850.036.6824332
GCGGCAC48550.035.571433
TCGCCGG46400.035.1917848
CCCGTCG47400.034.36543744
CGGCACC50300.034.29858434
CCGTCGC47700.033.89961245
TATCTCG4200.033.0697136
GAATTCG4450.032.5269629
TCGTATC4150.032.52416233
AATTCGT4750.031.9631230
ACGAATT4700.031.4591727
ACCCGCG54350.031.3802925
CCCCCGA55200.030.82652719
GCACCCC57150.030.62202836
CCGCGCG57100.030.23042527
CCCCGAC56800.030.04582220
TATGCCG4900.029.99139443
CACCCCC58250.029.84193837
CGAATTC4850.029.80982628