FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359274
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN282760.8417294927415865No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA71620.2132008285123512No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC58250.17340056214527305No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC45000.13395751582038262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT32850.047.2919129
TGGCGCG33250.046.61584515
CTACTCG38200.042.03141437
CCGAACT5300.041.63565467
TACTCGG38900.041.22877538
CGCGGTG38950.040.06002810
ACTCGGG42100.038.71033539
AGGATCG39950.038.3915559
GTAGTCC42250.038.2458127
GATCGCT40250.038.01503461
TAGTCCC42950.037.6648228
TGTAGTC43550.037.3013226
CGGGTAG4700.037.183981
GGGGTAG14300.037.1431921
CGTGCCT43850.036.5780420
GCCGGTT5900.036.2940863
GGGGTAT15350.035.94191
CCTGTAG45800.035.3204224
ATCGCTT43450.035.09343362
CGGTATA2150.035.064511