FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437557
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG208380.6061863119651543No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205010.5963828381609382No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG102580.29840959728085964No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62520.18187334784557754No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT57110.1661354269907379No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG56330.16386637370667598No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA52660.15319018710089752No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC51320.14929206992058605No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT50920.14812845285183635No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG44000.1279978775624666No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC40870.11889257399950023No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA36680.10670368520434716No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG36240.10542370642872248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2850.037.85014340
CGCGGCA61500.037.49313432
GCGGCAC62850.036.60540833
TCGCCGG61600.035.60468348
CGGCACC66000.034.99114634
CCGTCGC63400.034.7682245
CCCGTCG64100.034.72902344
ACCCGCG71050.032.36699725
CCCCCGA71950.032.1734819
GCACCCC73850.031.65149536
CCCCGAC74350.031.06694620
CACCCCC75850.030.84667637
CCCCCGT73700.030.09036342
GTTATCG2500.030.0715266
CCGCGCG77350.029.8671127
GCGCGGG77050.029.11181662
GGCACCC80800.028.77289835
TATCGGA2500.028.7046388
CGTCGCC77500.028.51341446
GTACGGC5900.028.38153814