FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437557
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198900.5786085874357866No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA111210.32351463553913434No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC64010.18620782142667017No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC44140.128405143536529No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34860.10140922754153603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT33150.047.622279
TGGCGCG36350.043.4299415
CGCCGTA2300.042.97266853
CGGCATA2100.042.56971
CCGAACT5600.041.80182367
GGGGACT40000.040.57221
CTACTCG39750.040.34591337
TACTCGG40050.040.15960738
ACTCTTC39700.040.0257535
GGACTCT42300.039.746763
TCTGGTC39400.039.20070310
ACTCGGG42300.038.71498539
GTAGTCC40900.038.6360127
AGGATCG40150.038.1594659
TGTAGTC42150.037.8606826
GGGACTC42000.037.844382
TAGTCCC42700.037.36010728
CTGGTCC41700.036.9566111
GATCGCT41900.036.56261461
TTCTGGT43050.036.4326869