FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3426714
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN200670.5856047513740569No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA102850.30014176846973517No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC60980.17795474031389838No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC42090.12282904263384688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT34600.046.4296079
TGGCGCG37800.042.67927615
GGGGACT39050.042.539431
CTACTCG38200.042.3585637
TCTGGTC35900.042.27287310
TACTCGG38550.042.0059438
ACTCTTC38850.041.175085
GGACTCT42000.040.2017063
CCGAACT5750.040.0790467
GTAGTCC41800.039.125427
ACTCGGG42450.039.0874639
TAGTCCC41950.038.84836228
CTGGTCC39450.038.20892311
GGGACTC41850.038.150752
AGGATCG39650.037.9714259
TTCTGGT40350.037.695529
GATCGCT40650.036.93811461
TGTAGTC44700.036.86626426
CGGGACT10950.036.5098041
GGGGTAT12150.036.307751