FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132504
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC49300.1573820815552031No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43260.13810038231395716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT27950.046.360029
TGGCGCG28700.045.63083615
CGCGGTG31400.041.8178810
CGGCAAT4500.040.0740281
TACTCGG33550.039.09007638
CTACTCG33800.038.68464737
GTACGCA7200.038.6844365
CGGCATA3000.038.1473961
CGGGTAG4450.037.4070471
TACGCAC7500.035.79915666
CGGTTAC1550.035.798141
GCGGTAT1850.035.616731
ACTCGGG37700.035.3517139
ACGCACG7150.035.27294567
AAGTACG8400.035.18499463
CCGAACT5600.034.8870367
AGTACGC8500.034.7999364
GTGGCGC38600.034.6437414
GTAGTCC37800.034.5973427
CGGCAAA6550.034.414721
Produced by FastQC (version 0.11.5)