FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115707
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG103070.33080774283332803No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG50510.16211408839149508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA33150.033.8661532
GCGGCAC35150.031.9479533
AGGACCG6150.031.75242870
TCGCCGG33150.031.74913448
CCGTCGC34050.031.07539245
CCCGTCG35050.030.5696944
CGGCACC37350.030.25904834
CGTACGT1600.030.2540319
ACCCGCG40100.028.55683925
GCACCCC40150.028.42206236
CCCCCGA41350.027.7611719
CGTTGCG1750.027.6830731
AGTCCGC43250.026.7689637
CAGTCCG41650.026.71866
CACCCCC43900.026.08078837
CCCCGAC43650.026.06242220
CCCCCGT41800.025.44494442
GTCCGCC45600.025.3894218
ACGTACG2350.025.35016457
GGCACCC45700.025.1919735
Produced by FastQC (version 0.11.5)