FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206210
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG106180.33116982356115165No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG51900.16187336450201328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA33100.034.02325432
GCGGCAC35000.032.1853433
TCGCCGG34650.030.8746248
CGGCACC37150.030.61214434
CCGTCGC35050.030.3788845
CCCGTCG35350.030.10640544
GCACCCC41450.027.36737436
ACCCGCG42850.026.97528825
TAGCGTC6950.026.85530911
CCCCCGA41800.026.79900719
GTTCCGT2850.026.7170811
GTAGCGT7200.025.9228310
CGTAGCG7200.025.922839
AGTCCGC45450.025.4760517
CGCCCTA10550.025.29974461
CCCCCGT42000.025.23970442
CAGTCCG44050.025.1087596
CCCCGAC44350.025.10614620
CACCCCC45800.025.00430937
CGTACGT1801.2732926E-1124.9701319
Produced by FastQC (version 0.11.5)