FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179069
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG104470.3286182212465348No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG49070.1543533657180766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA31950.035.57530632
GCGGCAC34000.033.33629233
CCGTCGC32650.032.50790445
CGGCACC34550.032.41199534
TCGCCGG33250.032.06558648
CCCGTCG34100.031.31581144
CGCCCTA10650.029.35284861
CCCCCGA39700.028.82959619
ACCCGCG40850.028.12832325
AGGACCG7450.027.66821570
GCACCCC40750.027.5817936
AGTCCGC42750.026.4404667
GTGCCGT5050.026.055671
CCCCGAC43850.025.94669520
CCCCCGT41050.025.91353442
CAGTCCG41550.025.7066176
CACCCCC44500.025.1087137
GGCACCC44900.025.0241735
TCCGCCC46500.024.9028649
GTCCGCC45850.024.878958
Produced by FastQC (version 0.11.5)