FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179069
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC49010.15416463121750423No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43690.137430172166757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT28750.046.9890759
TGGCGCG30350.044.73957415
GTACGCA6750.044.3730665
CGGGTAG4600.042.233341
CGCGGTG33200.040.89962410
CGGCAAA6400.039.0281331
AGTACGC7750.038.6152764
CTACTCG36700.037.80809837
TACGCAC7750.037.8079466
ACGCACG7350.037.66813367
TACTCGG36850.037.666738
AAGTACG8500.036.4138863
CGGCAAT3600.035.655331
ACTCGGG39700.035.3234639
CCGAACT5450.035.1693367
CGGCATA2300.034.6916731
GGGGGGA63200.034.6367761
CGTGCCT40450.034.44001420
GTAGTCC40150.034.39032727
TAGTCCC40450.034.31611328
Produced by FastQC (version 0.11.5)