FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029582
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG204550.5076208897101485No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG105910.2628312316265062No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG65600.16279604187233315No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG58130.1442581389335172No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC56380.13991525671893512No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA56190.1394437437927805No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG52350.12991421939049758No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC50140.12442977956522538No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC49370.12251891139080925No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA47320.11743153508229885No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC46900.11638924335079916No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46830.11621552806221587No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC46520.11544621749848992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG10350.036.93352570
CGCGGCA66000.034.43293832
GCGGCAC66550.034.41940733
CCCGTCG64450.033.47650544
TCGCCGG64950.033.22461748
CCGTCGC65600.032.81278245
CGGCACC70350.032.6252834
CGTACGT3150.031.73684719
GCACCCC75400.030.55754736
GTACGTA3550.030.10786220
CACCCCC78350.029.42097737
TACGTAG3550.029.1431621
CCCCCGA80250.028.95284319
GTGCCGT5850.028.8933141
CGCCCTA24450.028.64803561
ACCCGCG82050.028.18606225
GGCACCC82650.027.9071335
GTTCCGT3300.027.1779421
CGATCTA3600.027.13119547
CGTTCGT3200.026.9492821