FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029582
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC69310.17200295216724712No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA59390.14738501412801625No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC55810.13850071794047125No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC53760.13341334163196084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46550.11552066690788275No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC45080.11187264584763383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA2850.046.0791361
GCGCGGT37000.045.3474249
AAGTACG12750.042.27333563
TGGCGCG39500.042.2146215
TCTACGG5000.042.03255
AGTACGC12850.042.00290364
CCGAACT7050.041.15172267
GTACGCA12400.040.15357265
GTTGATC14750.039.93849610
CGGTTCA7500.039.046585
GGGGTAG17750.038.3560141
GTCTACG5500.038.211364
CTACTCG44050.037.53428637
TACGCAC12900.037.304466
TACGGCC5450.037.29767
CGCGGTG45400.037.2574610
TACTCGG45050.037.2555438
CGGGTAG5400.037.1193051
TAGCATA15950.036.77895724
GTAGTCC45150.036.71430227