FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085107
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG203300.4976613831657286No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG106700.2611926688823573No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG65800.16107289233794855No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG57790.14146508279954478No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA55110.13490466712377422No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC54650.13377862562718676No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG52870.12942133461865257No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC50550.12374216880977659No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC48950.1198255027346897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48490.1186994612381022No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC47090.11527237842240118No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA46460.11373019115533571No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC44530.10900571270226213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT2750.033.85606819
AGGACCG9600.033.6543970
TAGTCCG3900.033.584446
CGCGGCA69350.032.7831132
GCGGCAC69550.032.69849833
CGGCACC72500.031.3329934
TCGCCGG67650.031.16547848
CCCGTCG68600.030.84591144
CCGTCGC68550.030.73778245
GCACCCC76400.029.75441736
TATCGGA2700.029.3618378
CGCCCTA23350.028.60802561
CACCCCC80250.028.42697137
GTTCTCG26000.028.23536913
CCCCCGA81500.027.6287419
GTACGTA3500.027.59150320
GGCACCC83400.027.20543335
CGATCTA3350.027.06731847
GTCGTAC6400.026.92831411
ACCCGCG83900.026.9138325