FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717138
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA193300.5200237386935863No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC135240.3638283001599618No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC110980.2985630342483922No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA82690.22245609390880833No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69100.18589570793443772No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41620.11196786344763095No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA39800.10707162338336644No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC38430.10338599212620031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC8650.054.221997
CCGAACT10350.053.41181667
GCGCGGT68300.053.3719149
TCTACGG8950.052.0177575
GGGGACT54000.051.3342441
CGCGGTG73200.049.799210
TGGCGCG73550.049.5126715
GTCTACG9600.049.214184
GCCGGTT11350.048.612453
TCTGGTC61050.048.5815410
ACTCTTC61200.048.4605035
GGACTCT63800.047.3485643
TTCTGGT63400.046.672019
CGGTTCA11900.046.6573265
GGGACTC63300.046.5240482
CGAACTT12500.046.0879568
GCGTCTA1950.045.9792752
TGGTCCG20350.045.92526212
CTACGGC10300.045.534716
CGGGACT12750.045.3855131