FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699854
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA192000.5189393959869768No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC136710.3695010667988521No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC110160.2977414784475279No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA82060.22179253559735057No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT67510.1824666594952125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42840.11578835272959419No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38860.10503117150028082No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37420.1011391260303785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT58550.053.7421539
GGGGACT55100.051.1582341
TCTACGG7300.051.045775
GTCTACG7350.050.2290884
CGGGACT13450.050.0714951
ACTCTTC63550.049.4608655
TCTGGTC62750.049.4302810
CGCGGTG64650.049.0449310
TGGCGCG64100.048.9806515
GGACTCT65950.048.707263
GGGACTC65050.048.015082
CCGAACT10050.047.86017267
TTCTGGT66100.047.1339049
GCCGGTT10450.046.5545043
TGGTCCG22200.046.15775712
CGGTTCA10650.045.680245
TACGGCC8300.045.310767
TCTTCTG69650.045.1284337
CTGGTCC69300.045.0570211
CTCTTCT71700.044.031236