FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3718650
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA181380.4877576539873341No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC133000.3576566764820566No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC108450.29163809446976724No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA78760.21179729202802094No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT68110.18315786643002166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42750.11496107458351822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT63950.053.5142369
GGGGACT55900.051.6550521
TACGGCC8250.051.0150457
TCTACGG8350.050.8179175
ACTCTTC64750.050.1358155
CCGAACT11900.049.99839867
TCTGGTC64150.049.69052510
CGCGGTG69250.049.46838410
GCCGGTT12350.048.601683
CGAACTT12600.048.58307368
TGGCGCG70550.048.310415
TTCTGGT66750.048.168479
CGGTTCA12200.048.071465
TGGTCCG20100.048.0554512
GGACTCT67400.047.8542753
CGGGACT13150.047.0913051
GTCTACG9100.046.6264884
GGGACTC68050.046.393962
CTACGGC9250.046.2464266
TCTTCTG71100.045.560497
Produced by FastQC (version 0.11.5)