FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124461
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA151200.48392346711960876No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC117410.3757768139848761No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC91470.292754494295176No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86670.27739184454534715No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA67200.2150770964976039No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT55700.17827074813863894No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA32850.1051381342253912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT9750.053.1245867
GCGCGGT54750.052.2781229
GGGGACT47100.050.7189981
CGGTTCA10050.048.8902325
TGGCGCG58700.048.6353815
ACTCTTC52150.047.9655275
TCTGGTC51350.047.9106110
CGCGGTG61250.046.84243810
GCCGGTT10650.046.1284523
GGACTCT54900.046.118753
CGAACTT11600.046.0432868
GGGACTC54600.045.7436872
TTCTGGT53900.045.388979
TGGTCCG16550.044.6317412
CCGGTTC11200.043.563474
TGTAGTC67150.043.47031426
GTAGTCC67450.043.24602527
CTGGTCC56750.043.15829511
CGGCAAT2400.043.151461
TAGTCCC67900.042.97812728