FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161912
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA140100.44308633510357026No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC114920.36345097523270725No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC90490.28618759788381204No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85890.2716394384157434No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA61470.19440768750047438No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT54260.17160502885595802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT52500.052.505549
GGGGACT44150.050.046181
CGGTTCA8450.048.059355
TGGCGCG57800.048.0459715
GGACTCT48400.047.35373
CGCGGTG58650.047.35190210
CCGAACT8550.047.11618467
ACTCTTC48000.046.6041645
GGGACTC50000.046.2660142
GCCGGTT9050.046.0110133
TCTGGTC48850.045.5092710
TTCTGGT51750.043.1584939
TGGTCCG16150.042.61561212
CTACTCG65750.042.1892937
CGTGCCT66800.041.73929620
CGGGACT11400.041.684821
GTAGTCC67400.041.57301727
TAGTCCC67650.041.53409628
TACTCGG66850.041.46570638
CTGGTCC53900.041.3092611
Produced by FastQC (version 0.11.5)