FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604121
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC132820.36852258844805713No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC110330.30612179779757676No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA92180.25576277821971016No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC64020.17762999632920204No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT63440.17602072738401403No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG49230.13659363822690748No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT45400.12596691398540727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT63750.051.6412439
TGGCGCG67550.048.68020215
CCGAACT11300.048.09898867
CGGTTCA11700.047.338275
TACGGCC6350.046.882967
CGCGGTG70750.046.6786510
GCCGGTT11900.046.5407333
CGAACTT12700.045.18641768
TCTACGG6900.043.1447145
CTACGGC6900.043.1447146
CGGTATA1150.042.334761
CTACTCG78800.042.130737
TACTCGG79100.041.9328138
GTCTACG7250.041.5399064
GTAGTCC79550.041.49476227
TGTAGTC80200.041.19137626
CCGGTTC13450.041.179594
TAGTCCC80500.041.10093328
CGTGCCT79750.040.9526920
CCTGTAG81600.040.3890324