FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583304
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC133850.3735379415198934No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC111970.31247697655571505No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA91550.2554904635498412No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT64240.17927588616539372No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC63240.17648516564600714No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG49090.1369964702966871No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT46290.12918245284240468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT67100.053.1378979
TGGCGCG71150.050.3070915
CGCGTAA506.1278115E-849.0794261
CCGAACT10300.048.70055467
CGCGGTG74600.047.9348310
CGGTTCA10650.047.7845735
TCTACGG3900.046.159255
GCCGGTT11400.044.3371733
GTAGTCC83950.043.36549427
TAGTCCC83850.043.26391628
TGTAGTC84150.043.2551626
CTACTCG83400.043.24334737
CGTGCCT84600.042.77771820
TACTCGG84550.042.6218538
CCTGTAG86150.042.2350324
CGAACTT12200.042.0791268
CCGGTTC12300.041.092994
ACTCGGG88850.041.00199539
TACGGCC4350.040.5860377
AGTCCCA90100.040.07539729