FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593624
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC129080.359191724008967No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC107020.2978052239188073No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA87380.2431528729772508No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61510.17116426203743074No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC61030.1698285630327491No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG48010.13359772753076005No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT43950.12229994011616128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT63800.052.042239
CCGAACT10650.051.37236467
TGGCGCG67300.049.4310415
CGGTTCA11500.048.4734735
CGCGGTG71800.046.34009610
CGAACTT12550.044.87587768
GCCGGTT12350.044.8500143
GTAGTCC77500.043.35086427
TAGTCCC77750.043.30972728
CTACTCG77150.043.14590537
TACGGCC5550.043.0429467
TGTAGTC78950.042.67928326
TACTCGG78300.042.51994338
TCTACGG5550.042.4220925
CGTGCCT79600.042.1654120
CCTGTAG80800.041.6939524
CGGGTAG5000.041.1729241
CCGGTTC13700.040.9358674
ACTCGGG82900.040.715839
AGTCCCA84800.039.7997729