FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3097981
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN346901.119761547924277No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC78880.2546174427796684No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA78340.2528743720507001No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC62890.20300318174966211No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC46480.15003319904156934No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42430.13696016857430693No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT41340.1334417480287968No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG38430.124048533544912No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT33030.10661782625522881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT6750.054.57198367
CGGCATA1650.049.102051
GCGCGGT42000.047.9115039
GGGGTAG13050.046.3036041
TGGCGCG43400.045.97818815
TCTACGG5800.045.75745
CGGTTCA7600.045.086745
GTTGATC9700.045.0229410
TACGGCC6000.044.2321557
CGGTAAG2550.043.6863821
GTCTACG6200.043.347154
AGTACGC6900.043.1673164
TACTCGG48550.043.0181738
CTACTCG48500.042.9935737
CTACGGC6500.041.8633426
GCCGGTT8100.041.473453
GTAGTCC49750.041.33522827
CGCGGTG49050.041.29909510
CTTAGGC8200.041.08029618
TAGTCCC50450.040.8374728