FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077960
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN339991.1045952514002781No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC80320.2609520591560644No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA75700.24594211750640035No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC62510.20308905898712135No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47450.15416054789535927No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42470.13798100040286423No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT41430.13460213907913035No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG38840.12618747482098533No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT32220.10467972293337145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG4550.051.7056435
GCGCGGT39300.049.254469
CCGAACT7200.047.52800467
CGGTTCA7150.047.0051275
TGGCGCG42800.045.93406315
AGTACGC6350.045.73733564
GCCGGTT7400.044.508773
CGGGTAG3050.044.333191
TACGGCC5500.042.772567
GTTGATC9500.042.44956610
GTAGTCC47000.042.2164927
AAGTACG7400.042.13606663
CTACTCG47200.042.0950437
CGCGGTG46350.042.052710
GGGGTAG11550.041.8526461
GTACGCA5900.041.845465
TAGTCCC47250.041.7018228
TGTAGTC47550.041.44474426
TACTCGG48250.041.24466338
GTCGTAC1650.040.7377783