FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064475
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN340711.111805447915222No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC75590.24666541577268536No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA71350.23282944060565025No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC61180.19964267941490793No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45070.1470725001835551No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC40570.13238809257703196No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT39120.12765645012604115No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG38050.124164824317379No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT31780.10370454971895676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG6300.053.3361475
GCGCGGT42050.051.7793859
TACGGCC6600.050.3993347
CCGAACT6950.049.24338567
CTACGGC6800.048.920236
TGGCGCG45250.048.2589815
CGCCGTA2750.048.2193953
GTCTACG7300.046.9426734
CGGTAAG2200.044.630781
CGCGGTG49700.044.14735410
GCCGGTT7150.043.698593
CGTATCA3150.043.56935556
GCCGTAT3150.043.3554454
CGGTATA1250.043.3380281
GTTGATC9850.043.32268510
CTACTCG51150.043.14790737
CGGTTCA7250.043.1029625
TAGTCCC52000.042.93564228
GTAGTCC51950.042.8336427
TGTAGTC52050.042.74809626