FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036689
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC95420.31422381416075207No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC72710.2394384146680809No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA58740.19343436222807142No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT39990.13168948153729274No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG33590.1106138955948403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT47700.050.1934669
CGCGTAA651.8189894E-1048.5524561
TGGCGCG50750.047.17458315
CCGAACT6700.046.42943667
CGCGGTG52950.045.15143610
CGAACTT7550.042.00295368
TACTCGG57600.041.90760438
CTACTCG57600.041.89859437
GTAGTCC58850.041.0350227
TGTAGTC59900.040.48188426
TAGTCCC60550.039.95003528
ACTCGGG60850.039.67522439
GCCGGTT8000.038.9215853
CCTGTAG62450.038.5420124
AGGATCG61800.038.09210659
CGCATTA1009.094947E-1238.056662
GCTACTC63750.037.89979636
CGGGTAG4550.037.436581
CGGCAAT3750.037.080041
CGTGCCT64550.036.9491520
Produced by FastQC (version 0.11.5)