FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020994
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC96740.32022572702891827No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC71980.23826594822763636No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA57350.18983817908939904No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38840.12856695511477348No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG32460.10744807834772263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT44200.052.5127269
TGGCGCG47450.048.84225515
CGCGGTG49750.046.86311710
CTACTCG56150.041.6102937
GTAGTCC56550.041.52971327
TGTAGTC56800.041.33938226
TACTCGG56900.041.12830438
CCGAACT6800.041.0732267
TAGTCCC57400.040.8638728
CCTGTAG58400.040.19279524
GCCGGTT6800.040.1873783
ACTCGGG59550.039.8367439
CGGGTAG3100.039.303161
CGAACTT7350.039.14476868
CGGCAAT3300.039.0309331
AGGATCG59700.038.7980759
CGGTATA2100.038.126741
CGGCAAA6150.037.9245831
TCTACGG2650.037.854415
GATCGCT61700.037.74818461