FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015712
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG164230.5445811801657452No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62500.207247906961938No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46900.15551882938423828No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC42230.14003326577604228No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG36160.11990534905189885No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC31000.10279496185312126No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC30350.1006395836207171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA48900.038.15970632
GCGGCAC51000.036.3218333
TCGCCGG50500.035.1460548
CGGCACC53800.034.439534
CCGTCGC51850.034.12261645
CCCGTCG53050.033.60325644
GCACCCC56250.032.96251336
CACCCCC58150.032.02091237
CCCCCGA59000.031.78680219
TAGTCCG4000.031.1256666
ACCCGCG60250.031.0325525
CCCCGAC61400.030.49120
CGTACGT1950.030.16573119
CCGCGCG64100.029.23287227
GGCACCC63700.029.1473435
TCGTTTG16200.029.03739517
CCCCCGT62350.028.45846442
CGTCGTG1254.727728E-628.17556870
TAGCGTC1850.028.04113811
CTCGTTT16550.027.79171616