FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015712
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC93490.31000970914994536No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC69400.23012807589053597No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA54340.18018962022898738No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT37980.1259404081026305No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG30990.10276180218800735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA308.287183E-658.44699560
CGCGTAA352.0561713E-550.13118461
GCGCGGT45250.049.214189
TGGCGCG47400.046.68697715
CGCGGTG49150.045.449810
TACGGCC3250.044.687057
CGGGTAG4200.042.319951
CTACTCG53700.041.71029337
TACTCGG54550.041.00900738
GTAGTCC54800.040.6896227
TAGTCCC55300.040.3297728
TCTACGG3900.039.900485
TGTAGTC56850.039.33959626
ACTCGGG57750.038.992239
CCTGTAG58900.038.13278624
GTCTACG4100.037.9497034
CGGGACT7600.037.6031761
CGTGCCT59550.037.35173420
AGTCCCA60650.036.95643629
GCTACTC60900.036.8164136
Produced by FastQC (version 0.11.5)