FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316549
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133740.40325048717808787No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC124900.3765962752246386No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA102000.30754859946287544No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC87280.26316511530509573No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT58900.17759424027807216No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC39460.11897909543926533No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37930.1143658664473222No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG35800.10794352804677391No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG35210.10616457046164551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT63550.054.2573749
TACGGCC7850.053.2801257
TCTACGG8100.052.4760745
GTCTACG8350.051.3082924
CGCGGTG68150.050.84457810
CCGAACT8800.050.7048267
TGGCGCG69000.049.62084215
GCCGGTT9650.045.80573
CGGTTCA9700.045.5730365
GTAGTCC76850.044.98462327
TGTAGTC77500.044.96200626
CTACTCG77300.044.89188437
CTACGGC9400.044.8576
TACTCGG77550.044.70748538
GTCGTAC1750.044.688243
CGGCATA1850.044.4979741
TAGTCCC77950.044.4337728
GTTGATC10350.044.3536910
CGGGTAG4700.043.78791
CCTGTAG79200.043.68034424