FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596865
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54630.15188226413835382No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC46320.12877881154839008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT31150.046.5065659
TGGCGCG32700.044.40353415
CGGCATA3250.039.5518041
CTACTCG37950.038.95473537
CGGCAAA7500.038.9104231
CGGGTAG5450.038.8849221
CGCGGTG38350.038.3161510
TACTCGG38700.038.29963338
CCGAACT5800.036.0402767
CGGTATA1750.035.7340621
ACTCGGG42050.035.59053839
GTAGTCC41700.035.36420427
GGGGTAG17750.035.2307621
TACGGCC3300.034.574697
TAGTCCC43150.034.4256228
CGGCATT6650.033.9578061
AGGATCG42300.033.90109359
TGTAGTC44450.033.32650426
GGGGGGA73800.033.1408921
GATCGCT43950.032.7557361
Produced by FastQC (version 0.11.5)