FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3586217
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG138060.3849739154100268No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63510.1770946933774504No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37910.10571027910469444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA42500.036.88423532
GCGGCAC45000.034.92048633
TCGCCGG43700.034.2914348
CCCGTCG45500.033.2738444
CCGTCGC45700.032.82820545
CGGCACC48550.032.44534334
GCACCCC53100.029.73968536
ACCCGCG54450.029.06796325
CGCCCTA12050.028.83394261
CCCCCGA54300.028.48258819
CACCCCC56400.028.31640437
GGCACCC59700.026.73806835
CCCCGAC58300.026.47383520
AGGAGGG81100.026.28103370
AGTCCGC62800.025.3885157
CCCCCGT59450.025.21140142
CAGTCCG61800.024.903916
CGTCGCC62450.024.1448546
TCCGCCC67650.024.1307149
ACCCCCC66350.023.92241138
Produced by FastQC (version 0.11.5)