FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3586217
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54700.15252841643436524No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC47030.13114097668936375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT31000.045.9512759
TGGCGCG32050.044.4427515
CTACTCG37050.038.68898837
TACTCGG37200.038.5421638
CGCGGTG37750.038.37594610
CGGCATT6100.038.2119981
CCGAACT6400.038.18399467
CGGCAAT4400.037.9527551
TCTACGG3750.036.879995
GGGGTAG18300.036.501011
CGAACTT6900.036.1486268
ACTCGGG40900.035.74702539
TAGTCCC40850.035.69822328
CGGGTAG5100.035.4721831
GCCGGTT6750.035.3438153
GTAGTCC41200.035.30496627
GGCGAAT4450.035.1809271
TTAGCGG13300.034.8349510
CTTAGCG13500.034.5749869
TGTAGTC43100.033.8218626
Produced by FastQC (version 0.11.5)