FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587928
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG138120.38495755767674267No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63200.17614623258883677No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36980.10306784305593646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA44500.037.6384832
GCGGCAC46800.035.72262633
CGTACGT2350.035.33158519
TCGCCGG45050.034.66519548
CCCGTCG47150.033.50595544
CCGTCGC47350.033.1588545
CGGCACC50650.032.87630534
CGATCTA2700.030.97108547
CCCCCGA55600.029.80440319
ACCCGCG56150.029.66176225
GCACCCC56350.029.6276536
CACCCCC57700.029.18447137
CCCCGAC59050.027.89004320
GTACGTA3050.027.22535120
GGCACCC62300.026.90005335
CGTAGCA3350.026.86748923
GTCGTAC3750.026.74303611
AGGAGGG85950.026.24341270
AGTCCGC64950.026.0892097
CCGCGCG64800.025.92326527
Produced by FastQC (version 0.11.5)