FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587928
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50970.14205970688375016No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC45630.12717646507956681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT27650.047.39079
TGGCGCG29350.045.1131715
CGCGGTG33900.039.06142810
TACTCGG34550.038.48550838
CTACTCG34750.038.3526337
CGGGTAG6650.038.2571031
CGGCAAT3900.037.531471
CCGAACT6300.037.10462667
GTAGTCC37500.036.10149827
AGGATCG36300.035.04188559
GCGCATA1300.034.850651
ACTCGGG38850.034.68286539
TAGTCCC39250.034.23536328
TGTAGTC39850.034.224626
GATCGCT37600.034.15677361
CGAACTT7450.033.96173568
GGGGTAG17350.033.9467431
GCCGGTT7100.032.620213
CGTGCCT41450.032.2070720
CGGTTCA7300.032.2058755
Produced by FastQC (version 0.11.5)