FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617227
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67230.2568749290756973No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG55840.21335558589300813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA17800.037.75477232
GCGGCAC19150.035.29519733
CCGTCGC17600.035.2802145
TCGCCGG17550.035.2476648
CCCGTCG17850.035.16192644
ACCCGCG19800.034.5218225
CGCGATT501.8885029E-434.4173244
CGGCACC19850.034.06696334
CCCCGAC21600.031.26827220
CCGCGCG21950.031.00816327
CCCCCGA22150.030.48191819
AGTCCGC23550.029.3646057
CGTCGCC21550.028.99567846
CCCCCGT21700.028.73271642
CAGTCCG23700.028.017416
GCACCCC25100.027.66318136
CCCGCGC25350.027.38390526
GCGCGGG22850.027.34656762
GATTTCG3050.027.20391828
ATGGCCG6350.027.11817769
Produced by FastQC (version 0.11.5)