FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617227
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66800.25523196879750976No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC36890.1409507085170679No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG33830.12925894467694243No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC28100.1073655437606291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG8400.055.257144
TACGGCC8350.055.177327
CTACGGC8900.052.5391276
TCTACGG8900.052.152815
GCGCGGT22150.046.7234659
TGGCGCG22300.045.7906915
CGGTATG2950.045.6643751
ACGGCCA11300.041.381178
CGGTATT3600.041.257311
GAACGCG11050.040.8837724
CGGTACT2800.040.7090451
CGGCATA2900.039.305281
GCCATAC11900.039.2879511
CGGCAAT3000.039.146471
CCGAACT3750.038.92658667
CGGCCAT12100.038.645229
CGCGGTG27000.038.58523610
CTACTCG26700.038.4851937
TACTCGG26750.038.4385338
ATCTCGT11650.038.1016635