FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607531
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66760.25602763687181473No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG56250.21572130877830406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA15850.036.05982632
TCGCCGG16700.032.8590548
GCGGCAC17650.032.3955133
CCGTCGC16750.032.28684645
GTTCCGT2150.032.2481961
CCCGTCG17300.031.23784344
CGGCACC18750.030.5085734
ATGGCCG5750.029.97211869
ACCCGCG19800.028.5876125
TGGCCGT7750.028.38457170
CCCCGAC20350.026.90569520
CCCCCGA20500.026.86904519
CCGCGCG21550.026.45587327
CCCCCGT20900.025.6653342
CGTTGGA4700.025.06840333
GCGCGGG22350.024.95945762
GATTTCG3750.024.89160328
TACGGGT5250.024.8910146
GGGTACG5550.024.785273
ATTTCGT3800.024.57500829
Produced by FastQC (version 0.11.5)